Cytokine induction in human cord blood lymphocytes after pulsing with UV-inactivated influenza viruses.
Identifieur interne : 001665 ( Main/Exploration ); précédent : 001664; suivant : 001666Cytokine induction in human cord blood lymphocytes after pulsing with UV-inactivated influenza viruses.
Auteurs : Senad Divanovic [États-Unis] ; Alexander C K. LaiSource :
- Immunology letters [ 0165-2478 ] ; 2004.
Descripteurs français
- KwdFr :
- Alignement de séquences, Cytokines (immunologie), Cytokines (métabolisme), Données de séquences moléculaires, Humains, Lymphocytes (immunologie), Lymphocytes (métabolisme), Orthomyxoviridae (effets des radiations), Orthomyxoviridae (immunologie), Sang foetal (cytologie), Sang foetal (immunologie), Sang foetal (métabolisme), Séquence d'acides aminés.
- MESH :
- cytologie : Sang foetal.
- effets des radiations : Orthomyxoviridae.
- immunologie : Cytokines, Lymphocytes, Orthomyxoviridae, Sang foetal.
- métabolisme : Cytokines, Lymphocytes, Sang foetal.
- Alignement de séquences, Données de séquences moléculaires, Humains, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence, Cytokines (immunology), Cytokines (metabolism), Fetal Blood (cytology), Fetal Blood (immunology), Fetal Blood (metabolism), Humans, Lymphocytes (immunology), Lymphocytes (metabolism), Molecular Sequence Data, Orthomyxoviridae (immunology), Orthomyxoviridae (radiation effects), Sequence Alignment.
- MESH :
- chemical , immunology : Cytokines.
- chemical , metabolism : Cytokines.
- cytology : Fetal Blood.
- immunology : Fetal Blood, Lymphocytes, Orthomyxoviridae.
- metabolism : Fetal Blood, Lymphocytes.
- radiation effects : Orthomyxoviridae.
- Amino Acid Sequence, Humans, Molecular Sequence Data, Sequence Alignment.
Abstract
Mitogenic activity of UV-inactivated influenza viruses in cord blood lymphocytes (CBL), as measured by cytokine release, was investigated. Using prototype viruses of subtype H3N2 (A/Aichi/68), H2N2 (A/Japan/57), and H1N1 (A/Puerto Rico/34) for influenza A virus, and B/Lee/40 for influenza B virus, the results indicated that both Th1 and Th2 cytokines were induced. Stimulation indices were significantly higher for IFNgamma, IL-4 and IL-10 by influenza A viruses than by influenza B virus. Stimulation indices for IL-2 and IL-6 were lower, as these two cytokines were spontaneously released by cord blood lymphocytes in culture. Alignment of the amino acid sequences of the HA for the viruses used in this study indicated that influenza B virus lacked sequence homology to the antigenic sites identified for influenza A virus. Therefore, the antigenic sites may play a role in the mitogenic property, and cord blood lymphocytes could provide a system to compare this property for clinical isolates of influenza virus.
DOI: 10.1016/j.imlet.2004.05.004
PubMed: 15275967
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000328
- to stream PubMed, to step Curation: 000328
- to stream PubMed, to step Checkpoint: 000304
- to stream Ncbi, to step Merge: 000103
- to stream Ncbi, to step Curation: 000103
- to stream Ncbi, to step Checkpoint: 000103
- to stream Main, to step Merge: 001695
- to stream Main, to step Curation: 001665
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Cytokine induction in human cord blood lymphocytes after pulsing with UV-inactivated influenza viruses.</title><author><name sortKey="Divanovic, Senad" sort="Divanovic, Senad" uniqKey="Divanovic S" first="Senad" last="Divanovic">Senad Divanovic</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Molecular Genetics, Oklahoma State University, 306 Life Science East, Stillwater, OK 74078, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Molecular Genetics, Oklahoma State University, 306 Life Science East, Stillwater, OK 74078</wicri:regionArea><wicri:noRegion>OK 74078</wicri:noRegion></affiliation></author><author><name sortKey="Lai, Alexander C K" sort="Lai, Alexander C K" uniqKey="Lai A" first="Alexander C K" last="Lai">Alexander C K. Lai</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15275967</idno><idno type="pmid">15275967</idno><idno type="doi">10.1016/j.imlet.2004.05.004</idno><idno type="wicri:Area/PubMed/Corpus">000328</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000328</idno><idno type="wicri:Area/PubMed/Curation">000328</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000328</idno><idno type="wicri:Area/PubMed/Checkpoint">000304</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000304</idno><idno type="wicri:Area/Ncbi/Merge">000103</idno><idno type="wicri:Area/Ncbi/Curation">000103</idno><idno type="wicri:Area/Ncbi/Checkpoint">000103</idno><idno type="wicri:doubleKey">0165-2478:2004:Divanovic S:cytokine:induction:in</idno><idno type="wicri:Area/Main/Merge">001695</idno><idno type="wicri:Area/Main/Curation">001665</idno><idno type="wicri:Area/Main/Exploration">001665</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Cytokine induction in human cord blood lymphocytes after pulsing with UV-inactivated influenza viruses.</title><author><name sortKey="Divanovic, Senad" sort="Divanovic, Senad" uniqKey="Divanovic S" first="Senad" last="Divanovic">Senad Divanovic</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Molecular Genetics, Oklahoma State University, 306 Life Science East, Stillwater, OK 74078, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Molecular Genetics, Oklahoma State University, 306 Life Science East, Stillwater, OK 74078</wicri:regionArea><wicri:noRegion>OK 74078</wicri:noRegion></affiliation></author><author><name sortKey="Lai, Alexander C K" sort="Lai, Alexander C K" uniqKey="Lai A" first="Alexander C K" last="Lai">Alexander C K. Lai</name></author></analytic><series><title level="j">Immunology letters</title><idno type="ISSN">0165-2478</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Cytokines (immunology)</term><term>Cytokines (metabolism)</term><term>Fetal Blood (cytology)</term><term>Fetal Blood (immunology)</term><term>Fetal Blood (metabolism)</term><term>Humans</term><term>Lymphocytes (immunology)</term><term>Lymphocytes (metabolism)</term><term>Molecular Sequence Data</term><term>Orthomyxoviridae (immunology)</term><term>Orthomyxoviridae (radiation effects)</term><term>Sequence Alignment</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alignement de séquences</term><term>Cytokines (immunologie)</term><term>Cytokines (métabolisme)</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Lymphocytes (immunologie)</term><term>Lymphocytes (métabolisme)</term><term>Orthomyxoviridae (effets des radiations)</term><term>Orthomyxoviridae (immunologie)</term><term>Sang foetal (cytologie)</term><term>Sang foetal (immunologie)</term><term>Sang foetal (métabolisme)</term><term>Séquence d'acides aminés</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Cytokines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cytokines</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Sang foetal</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Fetal Blood</term></keywords><keywords scheme="MESH" qualifier="effets des radiations" xml:lang="fr"><term>Orthomyxoviridae</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Cytokines</term><term>Lymphocytes</term><term>Orthomyxoviridae</term><term>Sang foetal</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Fetal Blood</term><term>Lymphocytes</term><term>Orthomyxoviridae</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Fetal Blood</term><term>Lymphocytes</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cytokines</term><term>Lymphocytes</term><term>Sang foetal</term></keywords><keywords scheme="MESH" qualifier="radiation effects" xml:lang="en"><term>Orthomyxoviridae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Humans</term><term>Molecular Sequence Data</term><term>Sequence Alignment</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Alignement de séquences</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Mitogenic activity of UV-inactivated influenza viruses in cord blood lymphocytes (CBL), as measured by cytokine release, was investigated. Using prototype viruses of subtype H3N2 (A/Aichi/68), H2N2 (A/Japan/57), and H1N1 (A/Puerto Rico/34) for influenza A virus, and B/Lee/40 for influenza B virus, the results indicated that both Th1 and Th2 cytokines were induced. Stimulation indices were significantly higher for IFNgamma, IL-4 and IL-10 by influenza A viruses than by influenza B virus. Stimulation indices for IL-2 and IL-6 were lower, as these two cytokines were spontaneously released by cord blood lymphocytes in culture. Alignment of the amino acid sequences of the HA for the viruses used in this study indicated that influenza B virus lacked sequence homology to the antigenic sites identified for influenza A virus. Therefore, the antigenic sites may play a role in the mitogenic property, and cord blood lymphocytes could provide a system to compare this property for clinical isolates of influenza virus.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Lai, Alexander C K" sort="Lai, Alexander C K" uniqKey="Lai A" first="Alexander C K" last="Lai">Alexander C K. Lai</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Divanovic, Senad" sort="Divanovic, Senad" uniqKey="Divanovic S" first="Senad" last="Divanovic">Senad Divanovic</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001665 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001665 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= H2N2V1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:15275967
|texte= Cytokine induction in human cord blood lymphocytes after pulsing with UV-inactivated influenza viruses.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:15275967" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a H2N2V1
| This area was generated with Dilib version V0.6.33. |  |